
MTT assay, protein assay analysis
#1
Posted 15 April 2011 - 05:24 AM
#2
Posted 15 April 2011 - 05:25 AM
#3
Posted 15 April 2011 - 06:35 AM
If you have a curve with a known number of cells, you just have to create your equation with those values (value/Number of cells), and one you get the ecuation (your tendency line), you just introduce the values of your samples in that ecuation and you will have the number of surviving cells per sample
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"I´m the goddamn batman"
#4
Posted 19 April 2011 - 06:22 AM
With the MTT is easy, you just have to asign 100% to your positive control, and check what is the percentage of your treated samples. That is the easiest way, where you have the % of living cells respect your control. You can rest as well the value of the blanks if you want.
If you have a curve with a known number of cells, you just have to create your equation with those values (value/Number of cells), and one you get the ecuation (your tendency line), you just introduce the values of your samples in that ecuation and you will have the number of surviving cells per sample
Thanks
#5
Posted 19 April 2011 - 06:42 AM
#6
Posted 21 April 2011 - 02:04 AM
"This is SPARTA!"
"I´m the goddamn batman"
#7
Posted 22 April 2011 - 12:40 AM
you use to have number of living cells, instead of % of living cells respect a control (I am not sure if I understood your question)
I meant the first experiment we did was the MTT assay to provide a standard curve vs MTT activity, prior to doing the live/dead assay and the protein assay. A question that arose was why would we do the MTT assay first before the other two but I guess, like you said we would have needed a control for the experiment and if I am right in assuming, the MTT assay is the most accurate and precise of the three assays?, therefore the most plausible as the control assay?