1 reply to this topic

[mcb56]

I am doing a chromatin immunoprecipitation and I would like to compare the amount of protein I purify with my antibody to the amount of the same protein left over in my cell lysate. I can't use a loading control because the ChIP will only have my protein of interest and nothing else to compare it to.

Thank You

[ssmemo]

Did you figure out what type of control was appropriate? I'm curious too.