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Culture of mouse macrophages


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#1 Danillo

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Posted 14 April 2011 - 07:42 PM

Hi, I'm need of a protocol for peritoneal extration of mouse macrophages isolation and culture.
If you can send to my imail danillo_666@hotmail.com I thank

#2 newborn

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Posted 14 April 2011 - 10:58 PM

If you need monocytes, just inject PBS and retrieve the fluid. If you need activated macrophage, you can follow the paper by Parker (J Immunol 142 (1989), pp. 537542), and take out the fluid after 24-48hr instead of 6hr.

#3 Danillo

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Posted 15 April 2011 - 07:26 AM

And after this, how can I isolate and do a culture ?




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