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FACS sheath buffer composition


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5 replies to this topic

#1 Curtis

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Posted 14 April 2011 - 07:17 PM

anyone got the ingredients of the sheath buffer? can't find it at the BD website.

#2 Rsm

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Posted 15 April 2011 - 02:15 AM

That's because they want to sell it to you... I am not so sure, but I think it was just PBS with 0.01% NaN3. Or something like that. I will ask our old technician, she used to do it herself.
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#3 Curtis

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Posted 15 April 2011 - 07:05 AM

indeed, I also think it is PBS. I read a topic at Bioforum a while ago and the ingredients included Na2PO4 and stuff. it must be PBS, or a modified version

#4 Rsm

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Posted 15 April 2011 - 08:55 AM

Purdue Cytometry Forum, the best for stuff like that, sez: autoclaved standard PBS.
http://www.cyto.purd...v/2006/1856.htm
https://lists.purdue...ary/036478.html
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#5 lab rat

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Posted 16 April 2011 - 09:11 AM

Should you filter it before you use it? Tearing the tubing apart to remove a clog is a real nightmare.
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#6 Rsm

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Posted 16 April 2011 - 09:32 AM

So our former technician agrees: "Hi, Rsm. It's only autoclaved PBS. If you will culture the sorted cells, I think NaN3 seems toxic for the cells. In order to keep the inside of machine sterile, sometime you need to do the long cleaning with EtOH followed with protocol." Plus I agree with Labrat, you should definitely filter the PBS with sterile bottle top filter.
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