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iScript BioRad cDNA synthesis yield


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#1 krusty

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Posted 14 April 2011 - 09:10 AM

Hy forum fella,
This is krusty... new for you forum members, new for me. Useful tool! (I hope)
Is there anyone with an indication of the expected cDNA yield after a cycle of iScript cDNA synth. kit (BioRad)? I know it depends on RNA quality and quantity, but I only wanted to know if it is correct to expect a 100% transcription efficiency in the case of a "perfect" RNA sample, or if this percentage is lower. And, in the case of lower quality and low-concentrated RNA (c.a. 15 ng RNA), is it likely to expect very low efficiency? (e.g. 10% or lower)
Any help is appreciated, any reference as well.
Thanks in advance
Bests
K :unsure:

#2 krusty

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Posted 20 April 2011 - 02:01 AM

Still no one with an idea? Please!!! :unsure:




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