1-day old culture for DNA extraction - plausible?
Posted 14 April 2011 - 07:34 AM
I'm currently involved in a project that requires a large stack of growth bacterial plates (Salmonella) for DNA extraction. I am wondering if it is absolutely necessary to use a fresh culture for DNA extraction (if not, it would save me an two extra days)? The latest I will be receiving these bacterial plates is two days after they have been streaked and incubated, so they would only be one day old. It'd be helpful if you could also post your experiences in DNA extraction using non-fresh cultures. Thanks!
Posted 14 April 2011 - 08:45 AM
I don't extract the dna most of the time. I do direct colony/tip touch PCR.
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
Posted 19 April 2011 - 10:24 PM