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TE buffer in PEI transfection
1 reply to this topic
Posted 13 April 2011 - 01:04 PM
Does anyone know if using plasmid DNA dissolved in TE buffer will interfere with transfection of HEK293 cells using PEI? I know that the EDTA in the buffer can inhibit transfection with cationic lipid methods but am uncertain of its effects on transfection with PEI. Our lab usually uses plasmid dissolved in ddH2O, but TE buffer seems to be better for storage of DNA.
Posted 13 April 2011 - 03:29 PM
You could use 10 mM tris for storage, or TLE (tris low EDTA) with 1 mM EDTA instead of 10.