Edited by hianghao, 25 May 2011 - 06:54 AM.
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minimum replicate for qpcr?
15 replies to this topic
Posted 25 May 2011 - 06:50 AM
I am going to use Pfaffl formula for calculation. Should i sum up all replicates to get the mean then only substitute the value into the formula? But there might be differences in concentration of some biological replicates. Or i should do the calculation separately for each replicate, then only calculate the mean value?