My gene of interest shows tumor suppressor properties in vitro and also reduced tumorigenicity in vivo. I did these experiemnts using stable cell lines.
Now I want to check if my gene of interest reduces beta catenin via ubiquitination. I'm planning to treat my cells with a proteosome inhibitor and then check for beta catenin expression in the presence of my gene of interest. But I'm not sure which cell line to use? Do I demonstrate this in my stable cancer cell line which overexpresses my gene of interest or do I demonstrate this in the same cancer cell line but transiently or do i demonstrate this in HK293 cells?
Any advice would be appreciated.
what cell line to use
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