adrian kohsf, on 13 April 2011 - 03:20 AM, said:
wow... you people are super rich...
I still make my own master mix...
Well I do like 10 different genes a year, each has several exons, on just a small bunch of samples. If you need to optimise each and every single amplicon for just like 10 samples, you probably spent more money and more importantly time, than if you just set the first reaction and it works. If you have lot of samples for single assay, the situation may be different.
It was me who proposed using this mastermix in this lab, before that everyone was on cheap Taq (and troublesome Pfu mixes for sequencing) setting the reactions on ice (I hate to work on ice, you can possibly contaminate everything with the melted water) and still getting nonspecifities and things like that. And before they were using 50ul reactions, I started with 20ul, that saves some money too, and for a sequencing it's completely sufficient.
Sometimes expensive is not that expensive in the end.
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
I never trust anything that can't be doubted.