Fusion tagged protein and Western Blot problem
Posted 11 April 2011 - 10:01 AM
I have a Myc tagged protein. When I first received the plasmid, transfected, etc, I was able to see the Myc tagged protein band during Western, but now when I run it, I am not able to see the band. When I blot with the antibody raised against my specific protein, I am able to pick up the band with no issue.
Is there any reason why the myc Ab won't detect my myc tagged protein?
I thought I was diluting it too much but even with a small amount, I still can't get anything.
Any comment's would be greatly appreciated!
Posted 11 April 2011 - 03:47 PM
Posted 12 April 2011 - 05:07 AM
Posted 12 April 2011 - 03:32 PM