Sample preparation for ELISA
Posted 11 April 2011 - 04:57 AM
I have to test four proteins in human tear samples based on ELISA method and as you may be aware, I cannot collect enough tear fluid from human eyes. So is there any alternative to increase the initial amount of samples like with a diluent.
I would be very appreciated it if anybody recommend me with which solution and how can dilute human tear sample for ELISA experiments?
Thanks a lot
Posted 11 April 2011 - 06:11 AM
Posted 13 April 2011 - 01:38 AM
Which is the best dilution?
can I dilut with PBS?
Posted 13 April 2011 - 05:33 AM