Hello
I have to test four proteins in human tear samples based on ELISA method and as you may be aware, I cannot collect enough tear fluid from human eyes. So is there any alternative to increase the initial amount of samples like with a diluent.
I would be very appreciated it if anybody recommend me with which solution and how can dilute human tear sample for ELISA experiments?
Thanks a lot
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Sample preparation for ELISA
Started by Reza80, Apr 11 2011 04:57 AM
3 replies to this topic
#1
Posted 11 April 2011 - 04:57 AM
#2
Posted 11 April 2011 - 06:11 AM
you can always dilute samples using a sample diluent, however the signal depends on the amount of the tested protein in your samples.
#3
Posted 13 April 2011 - 01:38 AM
Thanks,
Which is the best dilution?
can I dilut with PBS?
Which is the best dilution?
can I dilut with PBS?
#4
Posted 13 April 2011 - 05:33 AM
A quick search on google indicates various buffers used in the litterature. I would probably stay with PBS...hope your assay has enough sensitivity. You can always increase the incubation time or incorporate polymers to increase sensitivity.