Agarose gel loading sample pops out
Posted 09 April 2011 - 11:28 AM
Posted 09 April 2011 - 11:30 AM
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
Posted 09 April 2011 - 12:35 PM
Posted 09 April 2011 - 01:46 PM
If you didnt do it that often its possible you made a rookie mistake: you pressed down the pipette till the end (to push out the last remains in your pipette tip, like you would normally do when using a pipette, push further down, further then the "first stop" on a pipette.. hard to explain, I hope you get what I mean?..) but when loading gels you cant do this because you would "blow" air into the well of your gel and thus "blow" the sample out of the well...
Not sure its this.. but I have seen this before many times...
If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.