I have a problem, I recovered my plasmid with kit and then I loaded it on an agarose gel with TAE1X and it poped out of the well, what happened?
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#2
hobglobin
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Posted 09 April 2011 - 11:30 AM
But you use a loading dye?
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hula
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Posted 09 April 2011 - 12:35 PM
Your eluted DNA may have residue ethanol. Make sure to spin off any ethanol before eluting the columns.
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pito
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Posted 09 April 2011 - 01:46 PM
How many times did prepare a loading gel?
If you didnt do it that often its possible you made a rookie mistake: you pressed down the pipette till the end (to push out the last remains in your pipette tip, like you would normally do when using a pipette, push further down, further then the "first stop" on a pipette.. hard to explain, I hope you get what I mean?..) but when loading gels you cant do this because you would "blow" air into the well of your gel and thus "blow" the sample out of the well...
Not sure its this.. but I have seen this before many times...
If you didnt do it that often its possible you made a rookie mistake: you pressed down the pipette till the end (to push out the last remains in your pipette tip, like you would normally do when using a pipette, push further down, further then the "first stop" on a pipette.. hard to explain, I hope you get what I mean?..) but when loading gels you cant do this because you would "blow" air into the well of your gel and thus "blow" the sample out of the well...
Not sure its this.. but I have seen this before many times...
If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.
