Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Trouble with H3-thymidineincorporation


  • Please log in to reply
1 reply to this topic

#1 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 16 November 2001 - 10:00 PM

When are you adding your thymidine?We had the same problem with stimulation experiments. If you add the thymidine from the beginning you dont get any labeling.

#2 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 16 November 2001 - 10:00 PM

Hi everyone:

I been trying to label exponetial L1210 cell, which is a mouse leukemic cell line, with H3-thymidine, but I just do not get any incorporation. Does anyone have a clue on what could possible is the problem? Thank you very much in advance.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.