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293 and MTT assay


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3 replies to this topic

#1 Sara

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Posted 16 October 2001 - 09:00 PM

Hi, I would like to know any protocol to assay MTT with HEK293T cells. Iīve proved, but formazan crystals donīt affix to the plate. Does anyone can help me?

#2 anonymous

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Posted 10 November 2001 - 10:00 PM

do you use 24 well plates or 96 well plates? Try bio-coated plates. Also try to suck off the media / mtt solution with mediaa pasteur pipette attached to a rubber bob..this usually worksand the crystals should be fine...

#3 Stephen Pratt

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Posted 15 February 2002 - 03:43 PM

I am using MTS, which produces a soluble formazan product. The assay is dead easy, just add cells, then add MTS solution (from Promega), incubate, and read.
As I undersatnd, the MTS does not enter the cells. It is reduced on the outside of the plasma membrane. One problem that I have come up against is that it is linear w.r.t. viable cell number only over a narrow range.

#4 buccalcells

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Posted 11 February 2009 - 10:26 AM

does anybody have a 500-1000 word document on structure physiology of buccal cells

or on the mtt assay ???? thank you




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