2 replies to this topic

[angel0071987]

Hi
I am quantifying DNA levels with nanodrop. I know that for "pure DNA" the 260/280 ration should be around 1.8-2.0 and that DNA absorbs at ~260nm. Typically proteins absorb at 280nm.
Can anyone give a list of things that absorb between 220 and 230nm?
This would be really helpful as on evert spectra there is a large peak at this value(i am not using phenol or chloroform so i dont think this could be an issue). I am using oragene kits to extract DNA from canine saliva.
is it possible for contamination from food particles to show up in this area?
Thanks in advance for all answers.    

[Maddie]

angel0071987, on 08 April 2011 - 03:40 AM, said:

Hi
I am quantifying DNA levels with nanodrop. I know that for "pure DNA" the 260/280 ration should be around 1.8-2.0 and that DNA absorbs at ~260nm. Typically proteins absorb at 280nm.
Can anyone give a list of things that absorb between 220 and 230nm?
This would be really helpful as on evert spectra there is a large peak at this value(i am not using phenol or chloroform so i dont think this could be an issue). I am using oragene kits to extract DNA from canine saliva.
is it possible for contamination from food particles to show up in this area?
Thanks in advance for all answers.    

Here is what little I know: "A230: Normally it's the indication of chaotropic salt contamination". I may have read that on this forum somewhere  

[hobglobin]

Read this too:
Link