agarose gel electrophoresis
Posted 08 April 2011 - 03:39 AM
After extracting DNA from whole blood,I resolved DNA on 0.8% agarose gel stained with ethidium bromide.After resolving,I visualized clear bands under UV light.Then I stored the gel in 1X TAE Buffer.After a period of 2 hours,bands disappeared.Can anyone please suggest me how to store agarose gel for atleast one day with intact bands?
Posted 08 April 2011 - 04:39 AM
How much TAE are you storing your gels in? I think you should be able to store it at 4oC (in a cling film with a little TAE) for a day. What do u need the bands for? Its intact DNA. Cant you use it from the tube directly ?
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Posted 08 April 2011 - 04:43 AM
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
Posted 08 April 2011 - 03:30 PM