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Sybr Green Real Time PCR - Melt curve


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#1 siuchi98

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Posted 08 April 2011 - 02:26 AM

Dear all,

Here is attached the melt curves of my SYBR Green Real Time PCR. I have run all the PCR products on agarose gel. All got single band. However, I'm not sure if these results are valid since some melt curves shown one major peak and also one or two very small peak(s). Any comment?

Thank you so much.

siuchi

Attached Thumbnails

  • Melt Curve 1.jpg
  • Melt Curve 2.jpg
  • Melt Curve 3.jpg
  • Melt Curve 4.jpg
  • Melt Curve 5.jpg
  • Melt Curve 6.jpg
  • Melt Curve 7.jpg
  • Melt Curve 8.jpg

Edited by siuchi98, 08 April 2011 - 02:31 AM.


#2 vladooo

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Posted 08 April 2011 - 03:51 AM

Yes, your result are valid, you have almost perfect melting curves. This is how melting curve should look like.

#3 ivanbio

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Posted 08 April 2011 - 05:35 AM

I agree with vladooo, very nice melt curves. In addition to melt curves though, you need amplification plots. As long as your amplification look like nice sigmoidal curves, you can assume your assay are working the way they should.

Ivan
Carlsbad, CA

#4 Maddie

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Posted 09 April 2011 - 06:12 PM

yeah I wish my melting curves looked like that :lol:
Theory is when we know everything and nothing is working. Practice is when everything is working and nobody knows why. Here, we combine theory and practice. Nothing is working and nobody knows why.

A. Einstein




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