Here is attached the melt curves of my SYBR Green Real Time PCR. I have run all the PCR products on agarose gel. All got single band. However, I'm not sure if these results are valid since some melt curves shown one major peak and also one or two very small peak(s). Any comment?
Thank you so much.
Edited by siuchi98, 08 April 2011 - 02:31 AM.