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ChIP on sorted T lymphocytes


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#1 chipy

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Posted 07 April 2011 - 11:20 AM

Hi everyone,

I'm just starting to standarize a protocol for Chip'in T lymphocytes not having good results so far. I was just wondering if anyone could help me put with any protocol using T lymphocytes using few cells (100.000).

Thanks

#2 chabraha

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Posted 07 April 2011 - 11:51 AM

There are micro-ChIP protocols out there for small cell numbers. just google it and a few papers should pop up, in fact, I just read a new one published in NAR I think. People use these protocols for ~10,000 cells
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