So after months and months of trying to get this to work, I have finally reached the sequencing stage.. and i'm wondering how to do this..
I've sequenced the product using my forward primer but it doesn't seem to match up with my genomic sequence (bs treated invivo).. I've used Bi-q analyzer..any thoughts on this?
It doesnt match the expected PCR sequence product that methyl primer express gave me when I was designing my primers.
How can you validate that BS sequence amplification is in the right spot corresponding to the genomic reference sequence?
Edited by sri2010, 07 April 2011 - 07:42 AM.