I am going to be preforming a xenograft on rats in order to develop a brain tumor. However, I am unsure as to the best way to maintain the cells - alive and healthy - during the 45-60 minutes it takes to preform the surgery. Will the viability be severely affected if I leave the cells (RG2-an adherent cell line)in suspension in a 15 mL tube in their growth media (DMEM + 10% FBS) outside of the incubator for approximately 1 hour?
As always thank you in advance for your help!
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bob1
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Posted 06 April 2011 - 07:03 PM
They will probably be mostly fine, there may be some loss of viability depending on how they are treated during preparation.
Edited to say: I think you mean xenograft, which may be more help when you are writing this up...
Edited to say: I think you mean xenograft, which may be more help when you are writing this up...
Edited by bob1, 06 April 2011 - 07:05 PM.
