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CHIP Assay has pull down in IgG control

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#1 benrabbit



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Posted 06 April 2011 - 12:41 PM

I was doing CHIP assay on mouse MLE12 cells and human A549 cells.

Everything works great in A549 cells. But for MLE12 cells, the control and treatment had no difference, and even the IgG pulled down the DNA fragment. I increased the washing, decrease the total lysate amount, and it was still the same.

The Kit I was using was EZ CHIP from Millipore, and I did pre-clear the lysate with protein G.

Please give me some idea how to fix the problem. Thanks a lot!

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