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Why the pre-incubation?


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#1 Ilham

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Posted 06 April 2011 - 11:17 AM

Hi,

These are some steps from an experiment I did, but there is one step in particular which I don't understand the goal.

Here are the steps :
1: 1900無 of natural substrate Casein (0.1%) with Tris-HCl buffer (0.2M pH=7.2) poured in 4 tubes
2: 1st incubation (pre-incubation) for 10min at 37蚓
3: Add different volums of venom (of Cerastes cerastes) to each tube (0, 2.5, 5, 10 無)
4: 2nd incubation 10min 37蚓
etc ...

My question, why the first incubation (pre-incubation)?

#2 hobglobin

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Posted 06 April 2011 - 11:41 AM

Though I'm not a pharmacologist I guess it's for two reasons:
All samples should have the same temperature.
The experimental temperature should be similar to natural conditions (as it's a snake venom that is working against endothermic animals such as rodents).
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

#3 Ilham

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Posted 06 April 2011 - 01:26 PM

You guessed right, thank you for confirming ^^

#4 pito

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Posted 07 April 2011 - 01:09 AM

You guessed right, thank you for confirming ^^


How do you know? You asked your teacher?
Just being curious.

Edited by pito, 07 April 2011 - 01:25 AM.

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.





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