1 reply to this topic

[metta]

hi
i made a egfp fusion construct which is supposed to be expressed in mouse testis. i would like to visualize egfp in cryosections of testis and if necessary i also need to detect RNA expression in testis.

My question is:
1. do i need to fix the tissue in paraformaldehyde
2. any good protocol for such an experiment

thanks

best regards
murali

[bob1]

You should either fix the tissue or freeze it and do cryosections.  Either should work fine using standard proceedures, which you can find just about anywhere.