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EGFP detection in mouse testis


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#1 metta

metta

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Posted 06 April 2011 - 03:13 AM

hi
i made a egfp fusion construct which is supposed to be expressed in mouse testis. i would like to visualize egfp in cryosections of testis and if necessary i also need to detect RNA expression in testis.

My question is:
1. do i need to fix the tissue in paraformaldehyde
2. any good protocol for such an experiment

thanks

best regards
murali

#2 bob1

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Posted 07 April 2011 - 03:59 PM

You should either fix the tissue or freeze it and do cryosections. Either should work fine using standard proceedures, which you can find just about anywhere.




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