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X-gal stained tissue for IFC?


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#1 Rnotk

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Posted 05 April 2011 - 02:41 PM

I have few tissue samples from mouse with reporter gene (B-gal)

When I sample these tissue, I did X-gal staining to see the reporter expression and then fixed with PFA, and now they are cryo-block (OCT)

so the part of the tissue is blue but I want to do IFC on this tissue, so I am planning to use antibody anti-b gal.

have anyone done something similar? Will this work? or not

Let me know your opinion.

Thanks in advance

#2 bob1

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Posted 05 April 2011 - 04:35 PM

I haven't done it, but I don't see why it wouldn't work unless the B-gal fluoresces in some manner.

#3 Rnotk

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Posted 06 April 2011 - 10:08 AM

hi, bob1

Thats exactly what I am afraid of.
I will go ahead testing to see it works or not.

But thanks anyway




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