X-gal stained tissue for IFC?
Posted 05 April 2011 - 02:41 PM
When I sample these tissue, I did X-gal staining to see the reporter expression and then fixed with PFA, and now they are cryo-block (OCT)
so the part of the tissue is blue but I want to do IFC on this tissue, so I am planning to use antibody anti-b gal.
have anyone done something similar? Will this work? or not
Let me know your opinion.
Thanks in advance
Posted 05 April 2011 - 04:35 PM
Posted 06 April 2011 - 10:08 AM
Thats exactly what I am afraid of.
I will go ahead testing to see it works or not.
But thanks anyway