Posted 05 April 2011 - 12:52 PM
I am relatively new to the immunocytochemistry technique and I need to use them for my cells attached on 8 chamber slides. I am thinking of using fluorescence secondary antibody for detection but then realized I will be finished with the experiment on saturday and I don't know if I could really keep them for few days before observing them in the microscope? How stable are these fluorophores? If anyone could englighten me in this area I would be really grateful!
Posted 05 April 2011 - 04:06 PM
The fluorophores do fade quite quickly under normal fluorescent light tubes, and very quickly under the microscope - so be careful with the amount of light you let get onto the slides.
Posted 06 April 2011 - 09:39 AM
I am wondering too, my protocol calls for blocking at 37C for 1h. Do you happen to know if I can block overnight at 4 C instead?
Posted 06 April 2011 - 07:10 PM