Thank you very much for replying. I added sodium butyrate to TEB buffer (Triton X-100 0.5%; PMSF 2 mM; NaN3 0.02%; NaBu 5mM; made with PBS) for acetylated histone purification. Since NaBu is 1:100 diluted in the TEB buffer, I just added it directly to TEB without adjust the pH value of stock. It turned out that there almost no effect to the pH value of TEB buffer. So I don't bother to adjust the pH value of NaBu stock now. Thank you again!
mdfenko, on 06 April 2011 - 12:04 PM, said:
what other components are in the buffer? that can help you determine what counter ion (acid) will be safe for reducing the pH.