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help with cell dilution


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#1 VetMed

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Posted 05 April 2011 - 03:42 AM

Hi. This is very simple but I was never good with mathematics o.O

I need to dilute cells to get 200 000 cells in 200l of medium. Which is the way to calculate that?

Tnx

#2 bob1

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Posted 05 April 2011 - 04:02 PM

Use V1C1=V2C2 (where V= volume and C= concentration and 1 and 2 are initial and final respectively) for one suspension to another.

or
V=N/C (where V and C are as above, and N= number of objects you have (in your case cells)) for pelleted cells.

#3 VetMed

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Posted 06 April 2011 - 04:10 AM

Thanks mate. Im just getting into lab work so Im probably asking some questions with very simple answers. Im a vet on a doctorate but I have just too much lab work before strating animal trials and I fell like Alice in Wonderland :rolleyes: But im slowly getting into this, love it more by each day :)) Thanks once more, cheers!




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