I stained huh7 with annexin V
I got only one population
how do I know if the staining is not right or I do not have apoptotic cells?
thanks
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#2
Rsm
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Posted 04 April 2011 - 10:47 PM
How about your positive and negative controls?
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michalcn
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Posted 05 April 2011 - 08:41 AM
Rsm, on 04 April 2011 - 10:47 PM, said:
How about your positive and negative controls?
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Posted 05 April 2011 - 10:29 PM
michalcn, on 05 April 2011 - 08:41 AM, said:
Rsm, on 04 April 2011 - 10:47 PM, said:
How about your positive and negative controls?
For a negative control you can use an isotype-matched antibody, for a positive control anything that induces apoptosis, for example serum starvation.
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