Posted 04 April 2011 - 08:52 PM
I got only one population
how do I know if the staining is not right or I do not have apoptotic cells?
Posted 04 April 2011 - 10:47 PM
Posted 05 April 2011 - 08:41 AM
Posted 05 April 2011 - 10:29 PM
For a negative control you can use an isotype-matched antibody, for a positive control anything that induces apoptosis, for example serum starvation.