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annexin V


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3 replies to this topic

#1 michalcn

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Posted 04 April 2011 - 08:52 PM

I stained huh7 with annexin V
I got only one population
how do I know if the staining is not right or I do not have apoptotic cells?
thanks

#2 Rsm

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Posted 04 April 2011 - 10:47 PM

How about your positive and negative controls?
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#3 michalcn

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Posted 05 April 2011 - 08:41 AM

How about your positive and negative controls?

thats the problem I don't have, any suggestion

#4 Rsm

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Posted 05 April 2011 - 10:29 PM


How about your positive and negative controls?

thats the problem I don't have, any suggestion


For a negative control you can use an isotype-matched antibody, for a positive control anything that induces apoptosis, for example serum starvation.
I got soul, but I'm not a soldier




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