4 replies to this topic

[Rnotk]

I do viral transfection (retro) made with Plat-E cells,
and I normally use 0.22um filter to remove cell debris from supernatant
but I just found some papers using 0.45um filter instead.

which is better? or no change??

Let me know your opinion or routine.

Thanks in advance

[bob1]

For the virus it shouldn't matter, both filter sizes are much bigger than a virus.  0.45 will get rid of most bacteria I think.  A 0.22 um filter will get rid of all but mycoplasma.

[laurequillo]

In general people do not recomend the 0.22 filter (they specifically mention it in most protocols)because you reduce the titer of the virus big time.
I always use 0.45 filters (but I never checked what happens with the 0.22 filter)

[Rnotk]

thanks everybody
I will switch to 0.45

Thanks

[SciCell]

Yep. Me too. I always use 0.45 syringe filter.

laurequillo, on 04 April 2011 - 08:22 PM, said:

In general people do not recomend the 0.22 filter (they specifically mention it in most protocols)because you reduce the titer of the virus big time.
I always use 0.45 filters (but I never checked what happens with the 0.22 filter)