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Filter for viral transfection


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4 replies to this topic

#1 Rnotk

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Posted 04 April 2011 - 03:15 PM

I do viral transfection (retro) made with Plat-E cells,
and I normally use 0.22um filter to remove cell debris from supernatant
but I just found some papers using 0.45um filter instead.

which is better? or no change??

Let me know your opinion or routine.

Thanks in advance

#2 bob1

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Posted 04 April 2011 - 03:53 PM

For the virus it shouldn't matter, both filter sizes are much bigger than a virus. 0.45 will get rid of most bacteria I think. A 0.22 um filter will get rid of all but mycoplasma.

#3 laurequillo

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Posted 04 April 2011 - 08:22 PM

In general people do not recomend the 0.22 filter (they specifically mention it in most protocols)because you reduce the titer of the virus big time.
I always use 0.45 filters (but I never checked what happens with the 0.22 filter)
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#4 Rnotk

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Posted 05 April 2011 - 07:16 AM

thanks everybody
I will switch to 0.45

Thanks

#5 SciCell

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Posted 05 April 2011 - 10:48 AM

Yep. Me too. I always use 0.45 syringe filter.

In general people do not recomend the 0.22 filter (they specifically mention it in most protocols)because you reduce the titer of the virus big time.
I always use 0.45 filters (but I never checked what happens with the 0.22 filter)






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