Hi,
Is it possible to detect pri-miRNA from RNA-seq data ? Is there any studies on this subject?
Thanks,
N.
0
pri-miRNA sequencing
Started by NicoBxl, Apr 04 2011 04:29 AM
4 replies to this topic
#2
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Epigenetist
Posted 19 April 2011 - 08:05 PM
Yes you can, but probably the abundance of pre-miR is lower than mature ones. There are several recent studies of deep sequencing detecting small RNA and Ago-binding RNA, pre-miRNA has been identified.
#3
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Enthusiast
Posted 20 April 2011 - 12:17 AM
I'm not looking after pre-miR but at pri-miR ( the first step in the life of the miRNA )
#4
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Epigenetist
Posted 20 April 2011 - 07:14 AM
Sorry I missed that. I have not read anyone mentioning detecting of pri-miR. I believe similar to pre-spliced mRNA it is at very low abundance and detectable.
#5
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Posted 07 December 2011 - 10:34 PM
read this " Features of Mammalian microRNA Promoters Emerge from Polymerase II Chromatin Immunoprecipitation Data" but the technique might no be available in any lab
i think the best routine method is RACE,however i personally heve not been successful in doing a race,and it is really a challenge for me because i have found some rarely expressed mirs but i can not clone the endogenous expressed pri or pre form only just some low expression detection of mature form by real time.
hope guys to expand this forum further
i think the best routine method is RACE,however i personally heve not been successful in doing a race,and it is really a challenge for me because i have found some rarely expressed mirs but i can not clone the endogenous expressed pri or pre form only just some low expression detection of mature form by real time.
hope guys to expand this forum further
