Western: Mouse antibody on mouse tissue homogenates
Posted 02 April 2011 - 08:02 PM
Im a newbie and Ive been trying a western on some mouse tissue homogenates. Unfortunately, the only good monoclonal antibody there is, has been raised in mice! Whenever I try a western on these samples, I get a lot(!) of bands! Ive tried incubating the primary and secondary in milk, reducing both the primary and secondary concentrations, but I still see all the non-specific bands! The antibody works perfect on all the invitro samples derived from mouse cells. It also worked fine on the non-mouse tissue homogenates. Apparently, I was going through a post-docs notes and she managed to successfully try the same antibody on mouse samples (I don't know how)!! I've emailed her, but am still waiting for a reply.
Can someone help me.....how do I get some specific bands with a mouse antibody on mouse tissue homogenates!!
Posted 02 April 2011 - 11:54 PM
Posted 04 April 2011 - 04:16 PM
Posted 05 April 2011 - 06:08 AM
Here is the link to that topic, that person never responded to the topic to report if they found anything that helped them.
Edited by proteaMatt, 05 April 2011 - 06:12 AM.