Can the above mentioned strain be used for conjugation? Does it contain the tra gene to allow for transfer of plasmid?
I am interested in using pNJ17, but according to this website: http://plasmid.med.h...do?vectorid=267, the strain should not be used for conjugation. I find it strange since the plasmid already contain the RP4 mob gene for plasmid transfer, so using the strain should not cause any problem.
Any comment is appreciated.
Edited by Isy, 02 April 2011 - 07:24 AM.