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DH5 Alpha Lambda pir and Conjugation


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#1 Isy

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Posted 02 April 2011 - 07:24 AM

Hi!

Can the above mentioned strain be used for conjugation? Does it contain the tra gene to allow for transfer of plasmid?

I am interested in using pNJ17, but according to this website: http://plasmid.med.h...do?vectorid=267, the strain should not be used for conjugation. I find it strange since the plasmid already contain the RP4 mob gene for plasmid transfer, so using the strain should not cause any problem.

Any comment is appreciated. :)

Edited by Isy, 02 April 2011 - 07:24 AM.


#2 pito

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Posted 02 April 2011 - 08:29 AM

Check this: http://ecoliwiki.net...x.php/DH5_alpha
(see the specifics about the gens.. dont care about the lambda not being there, that is not important because the only difference between the one on the link and the lambda pir is the pir itself)


And if you still have questions.. ask them.

Edited by pito, 02 April 2011 - 08:59 AM.

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.





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