HTC116 cells detaching !!!!
Posted 01 April 2011 - 07:40 AM
Has anyone had this problem before???
My HCT116 cells keep detaching from the T75 plate. I usually wash the T75 with PBS before adding tryp-edta for passaging, but since last week some of them are being literally washed out by the PBS. It had never happened before.
Could it be a sign of over confluency in the plate? I passage them twice a week and I need to do a 1:22 dilution, because if I do anything more concentrated than that the plate is overconfluent in no time. ATCC recommends 1:8 passages but it has never worked for us.
Are my passages ok or should I do less diluted passages more frequently ??
Thanks for your help.
Posted 03 April 2011 - 03:44 PM
Your PBS doesn't contain EDTA (by accident)?
How long have you had the cells up?
Would (do) you still use them for experiments even though they are behaving differently to normal cells?
Posted 30 June 2011 - 08:37 AM