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6 replies to this topic
#2
phage434
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Posted 02 April 2011 - 10:46 AM
Transformation efficiency is 1-2 orders of magnitude worse. Any preparing the DNA from these strains is difficult and yields poor quality plasmid DNA.
#3
Rsm
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Posted 02 April 2011 - 11:10 AM
Searching the forum before asking helps: "BL21 strains are recA+ and recombination is common. Second, they are endA+, which means that DNA you purify during a miniprep is often damaged by endonucleases. " http://www.protocol-...osts/29238.html
I got soul, but I'm not a soldier
#4
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Posted 02 April 2011 - 11:29 AM
I just realized that above answer was given by a user called "phage434". Weird. 
I got soul, but I'm not a soldier
#5
perneseblue
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Posted 03 April 2011 - 05:56 AM
Rsm, on 02 April 2011 - 11:29 AM, said:
I just realized that above answer was given by a user called "phage434". Weird.
And how is that?
-Perneseblue
May your PCR products be long, your protocols short and your boss on holiday
#6
phage434
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Posted 03 April 2011 - 11:59 AM
Who'd trust that fellow, anyway.
#7
Adrian K
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Posted 03 April 2011 - 12:32 PM
phage434, on 03 April 2011 - 11:59 AM, said:
Who'd trust that fellow, anyway.
haha
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
