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Why shouldn't you clone using expression bacteria


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6 replies to this topic

#1 Ahrenhase

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Posted 31 March 2011 - 09:21 PM

...?

#2 phage434

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Posted 02 April 2011 - 10:46 AM

Transformation efficiency is 1-2 orders of magnitude worse. Any preparing the DNA from these strains is difficult and yields poor quality plasmid DNA.

#3 Rsm

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Posted 02 April 2011 - 11:10 AM

Searching the forum before asking helps: "BL21 strains are recA+ and recombination is common. Second, they are endA+, which means that DNA you purify during a miniprep is often damaged by endonucleases. " http://www.protocol-...osts/29238.html
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#4 Rsm

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Posted 02 April 2011 - 11:29 AM

I just realized that above answer was given by a user called "phage434". Weird. :ph34r:
I got soul, but I'm not a soldier

#5 perneseblue

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Posted 03 April 2011 - 05:56 AM

I just realized that above answer was given by a user called "phage434". Weird. :ph34r:


And how is that?
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#6 phage434

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Posted 03 April 2011 - 11:59 AM

Who'd trust that fellow, anyway.

#7 Adrian K

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Posted 03 April 2011 - 12:32 PM

Who'd trust that fellow, anyway.


^_^ haha
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..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434




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