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Unstable A260/A230 in RNA isolation


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#1 hongbo

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Posted 31 March 2011 - 01:21 PM

I am using Qiagen RNeasy Mini kit isolate RNA form rats kidney, sometimes the RNA isolated has a low A260/A230(usually also low A260/A280) value because of the high 230 absorbtion. (wavelength scanning with Nanodrop(Thermo Fisher) shows a absorption peak near 230nm). I am sure it is not because of my disruption or homogenization problem because using the same homogenate did RNA extraction, sometimes got a low A260/A230 while sometimes absolutely normal. What's more, if the A230 high, then the A260 will be low. I want to know why and what can I do to solve this problem? Thanks!

Hongbo

#2 Ahrenhase

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Posted 31 March 2011 - 09:23 PM

I am using Qiagen RNeasy Mini kit isolate RNA form rats kidney, sometimes the RNA isolated has a low A260/A230(usually also low A260/A280) value because of the high 230 absorbtion. (wavelength scanning with Nanodrop(Thermo Fisher) shows a absorption peak near 230nm). I am sure it is not because of my disruption or homogenization problem because using the same homogenate did RNA extraction, sometimes got a low A260/A230 while sometimes absolutely normal. What's more, if the A230 high, then the A260 will be low. I want to know why and what can I do to solve this problem? Thanks!

Hongbo


read this http://answers.yahoo...26155235AAi9J1h

#3 hongbo

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Posted 01 April 2011 - 06:04 AM

:rolleyes:
Thanks a lot!~



I am using Qiagen RNeasy Mini kit isolate RNA form rats kidney, sometimes the RNA isolated has a low A260/A230(usually also low A260/A280) value because of the high 230 absorbtion. (wavelength scanning with Nanodrop(Thermo Fisher) shows a absorption peak near 230nm). I am sure it is not because of my disruption or homogenization problem because using the same homogenate did RNA extraction, sometimes got a low A260/A230 while sometimes absolutely normal. What's more, if the A230 high, then the A260 will be low. I want to know why and what can I do to solve this problem? Thanks!

Hongbo


read this http://answers.yahoo...26155235AAi9J1h






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