A colleague of mine is about to dry 80-100ul of PCR product in a speed-vac to concentrate her DNA. She needs her DNA in 16ul.
I'm not sure why but it seems that people do not do that. Instead they use precipitation, concentrators like centricons or ultra4, or a kit like the Minelute, right?
Do you know why people do not dry their PCR product? I've heard about severe loss? Is that true?
The colleague is about to start, so I would really appreciate your input before it's too late.
PS: the PCR product will be used for next generation sequencing.
PS: the PCR product has already been cleaned-up with minelute so there is no primer and the salt concentration should be low.
Edited by Maddie, 31 March 2011 - 06:51 AM.