
Background variation
#1
Posted 31 March 2011 - 01:30 AM
Best,
Grad
#2
Posted 31 March 2011 - 04:27 AM
#3
Posted 31 March 2011 - 05:44 AM
What is your %CV of replicates? Could be assay variability, carry over, manual v. automated washing, insufficient washing etc.
%CV for replicates are all < 10%. I use an automated 4x washing.
#4
Posted 31 March 2011 - 08:27 AM
do you kill the color reaction or just read it?
if the reader does a row at a time and you don't kill the reactions (all at the same time) then the reaction continues during reading.
genius does what it must
i do what i get paid to do
#5
Posted 31 March 2011 - 09:30 AM
does your reader read the whole plate at once or does it read a row at a time?
do you kill the color reaction or just read it?
if the reader does a row at a time and you don't kill the reactions (all at the same time) then the reaction continues during reading.
Not sure about the reader (it's fairly old, probably going to buy a new one soon), but we do use a stop solution to kill the reaction.
Edited by Gradstudent78, 31 March 2011 - 09:37 AM.