I am now currently working on detection of mRNA level on Mycobacterium Smegmatis, are there any good choice for the housekeeping gene??? I cant search for a good one. Thank you.
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Posted 31 March 2011 - 11:30 PM
Hi, maybe its helpful. in this paper they used elongation factor Tu and they used it for mycoplasma detection but it could be that it also works as reference gene.
Störmer M, Vollmer T, Henrich B, Kleesiek K, Dreier J. Broad-range real-time
PCR assay for the rapid identification of cell-line contaminants and clinically
important mollicute species. Int J Med Microbiol. 2009 Apr;299(4):291-300. Epub
2008 Oct 15. PubMed PMID: 18926769
Störmer M, Vollmer T, Henrich B, Kleesiek K, Dreier J. Broad-range real-time
PCR assay for the rapid identification of cell-line contaminants and clinically
important mollicute species. Int J Med Microbiol. 2009 Apr;299(4):291-300. Epub
2008 Oct 15. PubMed PMID: 18926769
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