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DC T cell co culture


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#1 mun2lo

mun2lo

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Posted 30 March 2011 - 11:22 AM

Hi, I am new to the DC field and I am interested to set up a co-culture system where I load DCs with an antigen and then co culture them with T cells and restimulate the T cells with the antigen. I would like to assess cytokine production and perhaps CD69 expression.
If anyone has a detailed protocol this would be great!

Importantly, I don't want to use OVA and OT-I or OT-II cells but rather a genuine antigen that we study in the lab.
:-)
Thanks!




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