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HEK 293 Trypsin vs. only EDTA


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#1 rnazitto

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Posted 29 March 2011 - 11:32 AM

Hey All,
I was thinking since HEK 293 cells are not that adherent that I could only use EDTA in place of Trypsin+EDTA to remove the cells from the walls of my culture dishes (100mm Falcon). Has anyone had and positive or negative experiences with this. Thanks!

#2 Rsm

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Posted 30 March 2011 - 05:41 AM

5mM EDTA work very well to detach 293 cells, you don't need Trypsin.
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#3 rnazitto

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Posted 30 March 2011 - 11:27 AM

Thank you! :)




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