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[garciapp]

I'm attempting to isolate Salmonella from poultry sources.  I am enriching samples in Rappaport-Vassiliadis (RV) broth or Universal Preerichment broth, then further selecting on Brilliant Green Agar.  All of these should be selecting against any gram positive organisms also present.  I've got a very interesting wrinkled colony phenotype on my Brilliant Green Agar that I believe is gram positive based on further culturing on EMB/MacConkey plates.  Does anyone have expertise or knowledge of Brilliant Green or gram positive isolates that I might be picking up from poultry sources?  I am not trying to quantify the Salmonella, just looking for presence or absence.

Any help or tips would be greatly appreciated.

Thanks,
Preston

[Phil Geis]

Some mycobacteria grow on these media - relevant to your observation, avium on Brilliant green.