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Brilliant Green Agar- Salmonella isolation tips?


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#1 garciapp

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Posted 28 March 2011 - 06:30 PM

I'm attempting to isolate Salmonella from poultry sources. I am enriching samples in Rappaport-Vassiliadis (RV) broth or Universal Preerichment broth, then further selecting on Brilliant Green Agar. All of these should be selecting against any gram positive organisms also present. I've got a very interesting wrinkled colony phenotype on my Brilliant Green Agar that I believe is gram positive based on further culturing on EMB/MacConkey plates. Does anyone have expertise or knowledge of Brilliant Green or gram positive isolates that I might be picking up from poultry sources? I am not trying to quantify the Salmonella, just looking for presence or absence.

Any help or tips would be greatly appreciated.

Thanks,
Preston

#2 Phil Geis

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Posted 05 April 2011 - 02:43 AM

Some mycobacteria grow on these media - relevant to your observation, avium on Brilliant green.




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