I am curious why the threshold for 'differential change' in 2D gels experiments always select 1.5 or 2 fold change in addition to statistical difference. Specifically,
(1)Why were changes limited to either 1.5 fold/2-fold or greater?
(2)Could there not be a highly significant change of a smaller magnitude? How would you justify spots in this situation?
Submit your paper to J Biol Methods today!
Threshold for differential expression in 2D gels experiments
No replies to this topic