why phosphorylated protein relative to total protein?
Posted 28 March 2011 - 05:54 AM
Posted 28 March 2011 - 06:18 AM
I'm going to assume you are trying to show that a certain Treatment/Condition induces phosphorylation.
The reason to calculate the ratio between p-protein and total protein is to show that your treatment is specifically inducing phosphorylation and that the increase in p-protein is not due to differences in total protein (which could be due to induction of protein expression rather than phosphorylation).
Posted 28 March 2011 - 07:29 AM
Hi. Below is a paper describing the different scenarios of phosphorylation status change. Maybe u would find useful:
phosphorylation change-2D gels
Edited by barnacleman, 28 March 2011 - 07:30 AM.