I was wondering, why do i have to calculate the ratio between p-protein relative to total protein? many i have talked to just say: thats how you do wheen you present you data. The reason i ask, wheen i try to get my manuscript published someone in the editorial board points out, i must present my data in this way. But i dont see the advantage to present my data like this. Can you please give me you reflections on this
/ Bettan
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#2
almost a doctor
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Posted 28 March 2011 - 06:18 AM
bettan, on 28 March 2011 - 05:54 AM, said:
I was wondering, why do i have to calculate the ratio between p-protein relative to total protein? many i have talked to just say: thats how you do wheen you present you data. The reason i ask, wheen i try to get my manuscript published someone in the editorial board points out, i must present my data in this way. But i dont see the advantage to present my data like this. Can you please give me you reflections on this
/ Bettan
/ Bettan
I'm going to assume you are trying to show that a certain Treatment/Condition induces phosphorylation.
The reason to calculate the ratio between p-protein and total protein is to show that your treatment is specifically inducing phosphorylation and that the increase in p-protein is not due to differences in total protein (which could be due to induction of protein expression rather than phosphorylation).
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Posted 28 March 2011 - 07:29 AM
bettan, on 28 March 2011 - 05:54 AM, said:
I was wondering, why do i have to calculate the ratio between p-protein relative to total protein? many i have talked to just say: thats how you do wheen you present you data. The reason i ask, wheen i try to get my manuscript published someone in the editorial board points out, i must present my data in this way. But i dont see the advantage to present my data like this. Can you please give me you reflections on this
/ Bettan
/ Bettan
Hi. Below is a paper describing the different scenarios of phosphorylation status change. Maybe u would find useful:
phosphorylation change-2D gels
Cheers
Edited by barnacleman, 28 March 2011 - 07:30 AM.
