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why phosphorylated protein relative to total protein?


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#1 bettan

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Posted 28 March 2011 - 05:54 AM

I was wondering, why do i have to calculate the ratio between p-protein relative to total protein? many i have talked to just say: thats how you do wheen you present you data. The reason i ask, wheen i try to get my manuscript published someone in the editorial board points out, i must present my data in this way. But i dont see the advantage to present my data like this. Can you please give me you reflections on this

/ Bettan

#2 almost a doctor

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Posted 28 March 2011 - 06:18 AM

I was wondering, why do i have to calculate the ratio between p-protein relative to total protein? many i have talked to just say: thats how you do wheen you present you data. The reason i ask, wheen i try to get my manuscript published someone in the editorial board points out, i must present my data in this way. But i dont see the advantage to present my data like this. Can you please give me you reflections on this

/ Bettan



I'm going to assume you are trying to show that a certain Treatment/Condition induces phosphorylation.

The reason to calculate the ratio between p-protein and total protein is to show that your treatment is specifically inducing phosphorylation and that the increase in p-protein is not due to differences in total protein (which could be due to induction of protein expression rather than phosphorylation).

#3 barnacleman

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Posted 28 March 2011 - 07:29 AM

I was wondering, why do i have to calculate the ratio between p-protein relative to total protein? many i have talked to just say: thats how you do wheen you present you data. The reason i ask, wheen i try to get my manuscript published someone in the editorial board points out, i must present my data in this way. But i dont see the advantage to present my data like this. Can you please give me you reflections on this

/ Bettan


Hi. Below is a paper describing the different scenarios of phosphorylation status change. Maybe u would find useful:

phosphorylation change-2D gels

Cheers

Edited by barnacleman, 28 March 2011 - 07:30 AM.





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