Dear all,
after purification I'd like to test the function of my proteins. But the purified proteins have no known usable function.
So my question is, can I add antibodies to the protein (which concenration?) and then add the mix to protein expressing cells, to have a look if there is a binding competition? To have a hint if the structure is physiological?
Has anybody a more detailed protocol for this? Especially for incubation times and concentrations?
Or other ideas to test if the structure is OK?
Thank you in advance,
Benita!
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