after purification I'd like to test the function of my proteins. But the purified proteins have no known usable function.
So my question is, can I add antibodies to the protein (which concenration?) and then add the mix to protein expressing cells, to have a look if there is a binding competition? To have a hint if the structure is physiological?
Has anybody a more detailed protocol for this? Especially for incubation times and concentrations?
Or other ideas to test if the structure is OK?
Thank you in advance,
Protein activity test after purification using antibodies
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