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Molecular Weight from Light Scattering


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#1 VanderWall

VanderWall

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Posted 27 March 2011 - 12:25 PM

I found this tutorial question in a book, and I am familiar with DLS, but I can't seem to figure out this light scattering problem:

Posted Image

Using excel I plotted the KC/R versus C graph and found both the slope and the intercept.

Molecular Weight:
Intercept = 1/M
M= 1/Intercept
Intercept = 0.0000542
M = 18,450 g/mol

2nd Virial Coefficient:
Slope = 2B
B = Slope/2
Slope = 0.00136
B = 0.000068

Now this is where I am confused, they also gave lambda = 436 nm and the dn/dc = 0.196 cm^3/g and it lists them as other information that will be needed to solve the problem, which leads me to believe I might be missing a significant part to this.




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