Posted 26 March 2011 - 07:58 PM
pls tell me the recepie of substrate buffer for western blot. I m using HRP conjugate secondary antibody. currently I m using citrate buffer, H2O2 and DAB but it s not giving good result.
Posted 28 March 2011 - 12:31 PM
genius does what it must
i do what i get paid to do
Posted 29 March 2011 - 04:46 AM
you can add a little nickel to the substrate solution to make it a little more sensitive
thank u so mauch....