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western blot


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#1 kalindi

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Posted 26 March 2011 - 07:58 PM

hii every one

pls tell me the recepie of substrate buffer for western blot. I m using HRP conjugate secondary antibody. currently I m using citrate buffer, H2O2 and DAB but it s not giving good result.

Thanking you,
kalndi

#2 mdfenko

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Posted 28 March 2011 - 12:31 PM

you can add a little nickel to the substrate solution to make it a little more sensitive
talent does what it can
genius does what it must
i do what i get paid to do

#3 kalindi

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Posted 29 March 2011 - 04:46 AM

you can add a little nickel to the substrate solution to make it a little more sensitive



thank u so mauch....




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