I am working on novel biomaterials for delivery of siRNA, and the synthesis conditions involve a 5 minute period of sonication, alternating every 30s between duty cycles of 1 and 4 (max on the machine is 10) on ice using a tip. All soluble agents to be loaded into the vehicles (ie- siRNA) must be encapsulated prior to the sonication.
I know that sonication is commonly used to create a smear from large nucleic acids such as gDNA, but since siRNAs are already small, and my sonication power is relatively low, I am wondering if I will get significant amounts of degradation? Anyone have experience with this?
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siRNA shearing during light sonication?
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