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3 distinct bands of gDNA on gel


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#1 Samantha30

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Posted 23 March 2011 - 08:44 PM

Hi,

I was wondering if anyone could help me with my problem of getting three distinct bands of the gDNA when run on gel. I extracted the DNA from gram negative bacteria and got three distinct bands of 5 kb, 6 kb and 12 kb, when run on the gel. The gDNA which is around 21 kb is not visible. I am attaching the image of the gel.07.03.11 mdi CTGK kit.jpg

Thank you.

#2 phage434

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Posted 24 March 2011 - 04:42 AM

Likely your organism has multiple plasmids, but possibly fewer with alternative supercoiling. You can gel isolate the bands and try restriction mapping them, or try transforming them into a strain missing the plasmids.

#3 Samantha30

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Posted 24 March 2011 - 10:22 PM

Thank you.

I will try that. Hope it works.




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